IMIBIO-SL   20937
INSTITUTO MULTIDISCIPLINARIO DE INVESTIGACIONES BIOLOGICAS DE SAN LUIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Changes in the fatty acyl content of polymorphonuclear leukocytes by hypothyroidism
Autor/es:
CORIA MJ, MARRA CA1, GIMENEZ MS
Lugar:
Mendoza
Reunión:
Congreso; SOBIO 2010; 2010
Institución organizadora:
Sociedad de Biología de Cuyo
Resumen:
Changes in the fatty acyl content of polymorphonuclear leukocytes by
hypothyroidism
Coria MJ, Marra CA1, Gimenez MS
UNSL,
IMIBIO-SL, CONICET, 1 UNLP, INIBIO-LP, CONICET,
5700 San
Luis. Email: marie_449@yahoo.com.ar
The
thyroid hormones are important regulators of lipid metabolism. Our goal was to determine
if hypothyroidism in female Wistar rats (200 g) obtained by 0.1g/l propyl-2-thiouracil in
drinking water for a month, affects the relative content of fatty acids
of complex lipids from polymorphonuclear (PMN) leukocytes. Total lipids from PMN were extracted with
chloroform-methanol (2:1) by Folch method and redissolved in n-hexane. The PMN fatty acid
composition of complex lipids from was determined by capillary gas-liquid
chromatography (c-GLC) The fatty-acyl methyl esthers (FAMEs) were prepared using a capillary
column. Before injection into the c-GLC system, the
FAMEs were purified by HPTLC chromatography and patterns of the FAMEs were
identified by comparison of their relative retention times with standards
running in parallel. Relative mass distribution of each analysis was calculated
electronically by quantification of the peak areas. We observed a decreased amount of palmitic, stearic
and arachidonic acids, and an increased content of miristic and linoleic acid. The
unsaturation index increased in the HT group compared with controls. The hypothyroidism
causes important changes in the relative content of fatty acids in complex lipids of
PMN. These
lipid alterations should alter the physiology of these cells and therefore
modify the host defense mechanism.