Nepeta cataria aqueous extracts activity against Helicobacter pylori

ARCE, SILVIA; MATTANA, CLAUDIA; ACOSTA, GIMENA; ORTEGA, CLAUDIA; DALFOVO, CELESTE; GOMEZ, MARIA ROXANA; VEGA, ALBA

Mendoza, Argentina

Congreso; XXVIII Reunión Científica Conjunta de la Sociedad de Biología de Cuyo; 2010

Nepeta cataria L. (Lamiaceae) has been used for its carminative, antispasmodic, and sedative properties in folk medicine. Helicobacter pylori is a bacterial pathogen that persistently inhabit the human stomach; induces inflammation of the gastric mucosa that may progress into peptic ulcer diseases or cancer. The aim of this work was to evaluate the bioactivity of N. cataria against H. pylori. To achieve more stable solids with improved technological properties for pharmaceutical use, spray-drying (SE) and lyophilization (LE) were applied to N. cataria aqueous extracts. SEs were obtained using a BÜCHI 290 Mini-spray Dryer. The reference strain of H. pylori (NCTC 11638) and two clinical isolates were used for this study. The minimum inhibitory concentration was assayed by agar dilution method, according to Clinical and Laboratory Standards Institute. The in vitro antibacterial activity of N. cataria extracts in the range of 25 at 100 mg/ml was assayed. AMX, (0.05 mg/ml) was used as positive control. After 72 h for incubation at 37 °C under the microaerophilic condition, the inhibition zone was determined in diameter. The LEs (10% and 15%) were active and inhibited H. pylori with a diameter average of 15-18 mm and 27-30 mm at concentration of 100 mg/ml respectively. The SEs did not show antimicrobial activity in the investigated concentration. This study show that the lyophilized extract of N. cataria can inhibit the growth of H. pylori and can reduce the colonization of gastric mucosa.