PRODUCTION OF KOSAKONIA RADICINCITANS IN CULTURE MEDIA FORMULATED FROM BAGASSE OF BEER

LAMBRESE, Y.; BENUZZI, D.; POSSETTO PAOLA; CALVO JUAN; NAVARTA GASTÓN; SANZ FERRAMOLA, M.

San Luis

Congreso; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2019

Sociedad de Biología de Cuyo

In previous works we show the biotechnological applications of Kosakonia. radicincitans. That is why the need arises to develop an economic culture medium for its large-scale production. The increase in craft breweries currently allows for a by-product, such as bagasse. This residue of the production process is the filtering and pressing of malt grains. Currently in our country there are not many uses that are given to this waste. For this reason an alternative is sought for reuse and thus optimize the production of beer and K. radicincitans (Circular Economy). The objective of this work was to develop a culture medium with a novel substrate such as beer bagasse, for the production of K. radicincitans. Weighed 250gr of Bagasse 900ml of distilled water was added. It was processed to release the remaining sugars and sterilized. Two different liquid culture media were tested from beer bagasse, the first formulated with the addition of 5g / l yeast extract (a) and the second without the addition of any substrate (b). A base medium prepared from commercial components (glucose 10g / L; Yeast extract 5g / L) was used as control (c). A suspension of K. radicincitans was standardized to a concentration equivalent to the 0.5 Mc Farland turbidity scale. An inoculum of (0.1 ml of the bacteria suspension) was seeded in 100 ml of the respective culture media. It was incubated in an oven at 28 ° C with stirring of 120 rpm for 5 hours. Subsequently, to quantify the growth, serial dilutions were made and seeded on plates containing counting agar. The bacteria reached a growth of 7.8 x106 cfu / ml in the bagasse-based medium with the addition of yeast extract (a); the medium containing only bagasse the microbial density was 6.2 x105 cfu / ml. In the control trial (c) a development of 9.5 x106 cfu / ml was obtained. They did not show significant differences between the culture medium made from commercial components and formulated from the bagasse of beer with yeast extract. With these results we can affirm that an economic medium with bagasse can be formulated for the production of K. radicincitans. The possible alternatives to enrich this beer by-product and obtain a better production of the microorganism of interest constitute future research perspectives.