WHEN CADMIUM ATTACKS LUNGS: DIFFERENCES BETWEEN ANIMAL AND VEGETAL PROTEIN SOURCES IN DIET

MARTIN MOLINERO GD; FILIPPA V; GÓMEZ NN; BOLDRINI GG; PÉREZ CHACA MV; GIMÉNEZ MS; CIMINARI ME; MOYANO MF; ÁLVAREZ SM

Mendoza

Congreso; Reunión Anual de la Sociedad de Biología de Cuyo; 2018

Sociedad de Biologia de Cuyo

Cadmium (Cd) is a toxic metal and an important environmental contaminant. We studied its effects on morphology, bronchoalveolar lavages (BAL) and oxidative stress markers under different diets. 4 lots of female Wistar rats were used: 2 lots received casein (Cas) and 2 lots received soybean (Soy) as protein sources. Within each group: 1 lot received regular water (Co) and the other 15ppm of Cd in the drinking water for 60 days. BAL was performed and nitrites concentration was measured. Total RNA was isolated with Trizol and cDNA was obtained. SOD, GPx and Nrf-2 were determined by PCR. S28 was the control. Homogenates were made and TBAR?S and Catalase (CAT) activity were determined. Lungs were examined for evidence of injury and analyzed morphometrically. A lung section was mineralized and Cd, Cu, Zn, Se and Mn concentrations were measured by ICP-MS. Cd was higher in intoxicated groups, but only significant in Cas-Cd (p