EFFECT OF Ligaria cuneifolia ON DIURESIS IN RATS

MOYANO F,; WENDEL GH; SANTILLÁN J,; PETENATTI EM,; VILLEGAS GABUTTI CM, ; MARIA AO

Mendoza

Congreso; Congreso SBC 2018; 2018

Sociedad de Biología de Cuyo (SBC)

Ligaria cuneifolia (Ruiz & Pav) Thiegh. (Loranthaceae) is a widespread hemiparasitic plant in Argentine and it is employed as antihypertensive agent in folk medicine. It is well recognized that the diuretics are used for the management of mild to moderate hypertension. This study was designed to determine the diuretic activity of L. cuneifolia. The test was performed as described by Lipschitz et al. The experiments were carried out taking into account international guiding principles and local regulations concerning to the care and use of laboratory animals for biomedical research. The experiments were approved by the local Committee CICUA (Protocol F-300/18). The 10% infusion of L. cuneifolia was prepared according to Argentinean Pharmacopoeia. Wistar rats (200-230 g) were employed. The animals, randomly assigned into groups (n=7), were deprived of food for 24 hours prior to starting the experiments and had free access to water. The test groups were administered different doses of an infusion of L. cuneifolia lyophilized (250 or 500 mg/kg, orally) or furosemide as standard drug (10 mg/kg by intra-peritoneal route). The control group received only the vehicle (50 ml/kg, orally). At the end of the experiments, the animals were euthanized by inhalation of carbon dioxide. Urinary volumetric excretion (UVE), urine chemical parameters and urinary levels of sodium and potassium were measured in 3 hours diuresis. The urinary concentration of sodium and potassium were determined by inductively coupled plasmamass spectrometry (ICP-MS). All values were expressed as the mean ± SEM. Graph Pad Prism was used for the statistical analysis and p values less than 0.05 were considered statistically significant. Student?s t-test was performed to evaluate the differences between the control and the experimental samples for each time point. The lots treated with infusion of L. cuneifolia (250 and 500 mg/kg) showed diuretic activity (UVE infusion of L. cuneifolia 250 mg/kg group: between 45 and 180 min, p