Synthesis and study of pseudostationary phase based on carbon nanotubes for the separation of emerging pollutants. V.M. Lanaro, C.M. Peralta, P.W. Stege, L.L. Sombra.

P.W. STEGE; C.M. PERALTA; V.M. LANARO; L.L. SOMBRA

Ciudad de México

Simposio; 23st Latin American Symposium on Biotechnology, Biomedical, Biopharmaceutical and Industrial Applications of Capillary Electrophoresis and Microchip Technology; 2017

Emerging pollutants (EPs) are defined as synthetic or naturally occurring chemicals that are not commonly monitored but which have the potential to enter the environment and cause adverse ecological and(or) human health effects [1]. Therefore, continuous efforts are devoted to develop appropriate methods for their monitoring and quantification in environmental samples. Electrokinetic chromatography (EKC) has become in a widely accepted technique for electrophoresis-driven separations. The use of pseudostationary phase (PSP) in EKC benefits from not requiring packing or frits. PSPs have the advantage of introducing a novel interaction phase for the analytes, which greatly simplifies column exchange and circumvents contamination inherent with complex mixtures. The aim of this study was to develop a methodology to separate a mixture of emerging pollutants (pharmaceutical group). The studied analytes were paracetamol, naproxen, piroxicam, ketorolac, trimethoprim, sulfamethoxazole, cephalexin and amoxicillin. We performed an EKC methodology adding carbon nanotubes Carboxildos???? (c-MWNTs) to the BGE to generate a pseudo stationary phase PSP system. To reduce the sedimentation of c-MWNTs phenomenon we studied the addition of different additives into the BGE. Al menos el que quedo como finalA Beckman P/ACE MDQ instrument (Beckman Instruments, Fullerton, CA, USA) equipped with a diode array detector and a data handling system comprising an IBM PC and P/ACE System MDQ Software was used.The results showed that the interactions between the analytes and the MWCTs resulted in an increase of the migration time. Moreover and as expected, we could not achieve a good separation of the analytes with a fused silica capillary. Under the optimum conditions (20 mM sodium tetraborate, pH=9,2, c-MWNTs 2 %, voltage applied 12 kV, UV detection 190 nm) the drugs were separated in less than 25 min. The potential of EKC method was shown performing the analysis of water samples spiked with the studied compounds. As preliminary conclusions we can demonstrate the applicability of the EKC methodology for monitoring and quantification of EPs ?in water samples. [1] Geissen, V., Mol, H., Klumpp, E., Umlauf, G., Nadal, M., van der Ploeg, M., van de Zee, S. and Ritsema, C. International Soil and Water Conservation Research 3(2015)57?65.