On-line methodology for montelukast determination in bile salt medium with fluorescent detection. Multivariate optimization

PERALTA CECILIA; ACOSTA MARÍA GIMENA; HENESTROSA CECILIA; FERNÁNDEZ, LILIANA PATRICIA; LUCONI, MARTA OLGA

Rosario

Congreso; 4ta. Reunión Internacional de Ciencias Farmacéuticas; 2016

RiCiFa

Montelukast is strong and selective antagonist of the cysteinylleukotriene receptors used for the treatment of asthma. It is the first leukotriene inhibitor approved by the FDA for use by children [1]. In drugs quality controls, the development of simple, rapid and reliable methodsis required. In this context, flow injection analysis meets these requirements due to its speed and simplicity[2].In this work, a new on-line method has been developed for the determination of Montelukast using its native fluorescence (λex = 283 nm; λem = 397 nm) employing taurodeoxycholate as enhancing emission intensity agents. A multivariate study including a screening phase and a response surface method was used to optimize the system aiming to achieve high sensitivity and sampling frequency [3]. A two-level factorial design with 2(6-1) = 32 experiments are described here for the variables: Sample Taurodeoxycholate concentration, STDC (5×10-5 ? 2×10-4 mol L-1); HCl concentration (1×10-6 ? 1×10-4 mol L-1) and SDS concentration (1×10-3 ?¬ 2.5×10-4 mol L-1); eluent (5 ? 15 rpm) and sample (5 ? 15 rpm) flow-rates; while sensitivity and sampling frequency were the dependent variables. The eluent composition (HCl and SDS concentrations) did affect neither the sensitivity nor the sampling frequency, and they were fixed at 1×10-5 and 6.25×10-4 mol L-1, respectively. On the other hand, the sensitivity was negatively affected by eluent flow rate and positively by sample flow rate and STDC, whereas the sampling frequency was only influenced positively by eluent flow rate.A Response Surface Design was then built to optimize both analytical responses at once through the desirability function, obtaining 10 solutions where that with the highest desirability was: STDC= 5×10-5, Eluent Flow Rate = 5 and Sample Flow Rate = 14 rpm. This method is sensitive, simple and reliable and can be applied to analysis of pharmaceutical and environmental samples.References[1] S.M. Abdolraouf, B. Alireza, H.F. Mohammad, J. Fluorescence 26 (2016) 925?935.[2] M. Garrido, M.S. Di Nezio, A.G. Lista, M. Palomeque, B.S. Fernández Band, Anal. Chim. Acta 502 (2004) 173?177.[3] L. Vera-Candioti, A.C. Olivieri, H.C. Goicoechea, Anal. Chim. Acta 595 (2007) 310?318.