NEW METHODOLOGY FOR DETERMINATION OF ALUMINIUM IN URINE SAMPLES BY SOLID SURFACE FLUORESECENCE

TALIO MC; FERNANDEZ, L; SANTAROSSA, D

Mendoza

Congreso; XXXIV Reunión Anual de la Sociedad de Biología de Cuyo; 2016

Sociedad de Biología de Cuyo

Aluminum (Al) is a toxic metal, capable of interfering with a variety of cellular and metabolic processes of the nervous system and other tissues of the human organism. Al has been considered as a possible cause of renal osteodystrophy, Parkinson´s and Alzheimer´s disease. In this work, the separation/preconcentration of aluminium on nylon membrane (0.45 µm pore size) is carried out employing quinizarina (QZ) and SDS solution (acetic/acetate buffer pH 5) is proposed for later quantification by solid surface fluorescence (λem = 490 nm, λexc = 565 nm). Experimental parameters that influence efficiency of separative and determinative step were studied and optimized, like nature of membrane, nature and concentration of fluorophore, pH and buffer concentration. Working at optimal conditions, quantitative recoveries > 99.9%) were reached for both analytes with detection limits of ug L-1. Calibration of the new methodology presents a linearity of magnitude three-orders. The developed methodology was successfully applied to determination of Al(III) in urine samples. The proposed methodology represents an alternative to the routine metal analysis methods, with the advantage of using an simple inexpensive instrumental as a spectrofluorometer.