GREEN SPECTROFLUORIMETRY METHOD FOR BOLDINE ALKALOID DETERMINATION IN HERBAL DRUGS AND PHYTOPHARMACEUTICALS

HENESTROSA, CECILIA; ACOSTA, GIMENA; PERALTA, CECILIA; FERNANDEZ, LILIANA

Rosario

Congreso; 4ta. Reunión Internacional de Ciencias Farmacéuticas; 2016

RiCiFa

Boldine (BOL)is an alkaloid of the aporphine class that can be found in the tree leaf and bark of Peumusboldus Molina (boldo)[1]. This compound exhibits strong free-radical scavenger and antioxidant properties [2]. In addition, it has α-adrenergic antagonist activities in vascular tissue, and it has also been reported to have hepatoprotective, cytoprotective, antipyretic and anti-inflammatory effects.In this work, a new on-line method with fluorescence detection has been developed for the determination of BOL using its native fluorescence in acid media (λex = 282 nm; λem= 373 nm).Considering the need of studying simultaneouslydifferent aspects of the analysis and aiming to achieve adequate sensitivity and high sampling frequency, multifactor optimization was developed. The key factors selected during the optimization process were: sample pH; eluent composition (HCl concentration; SDS concentration); eluent (EFR) and sample (SFR) flow rates. Thesefactors were evaluated at two levels each. It could be concluded that the sensitivity and sampling frequency were negatively affected by HCl and SDS concentrations, and positively affected by sample pH and EFR. As it was expected, the sample flow rate did not affect the sensitivity significantly and because of that, it was fixed at 9.75 rpm.An adequate sensitivity (138.764 cps) and sampling frequency (26 samplesh-1) were obtained employing the optimal experimental conditions (sample pH = 3.0 and EFR = 7.5 rpm). The applicability of the proposed methodology was corroborated through BOL quantification in different real samples. The present methodology represents an eco-friendly alternative for the BOL quantification with simplicity and suitability. References[1] H. Speisky, B.K. Cassels, Boldo and boldine: an emerging case of natural drug development, Pharmacol. Res. 29 (1994) 1?12.[2] N. Quezada,M. Asencio,J.M. Del Valle, J.M. Aguilera and B. Gómez, Antioxidant activity of crude extract, alkaloid fraction, and flavonoid fraction from Boldo (Peumus boldusMolina) leaves,J. Food Sci. 69 (2004) 371?376.