PHOTOINDUCED METHODOLOGY FOR CARBAMAZEPINE DETERMINATION MEDIATED BY SODIUM TAURODEOXYCHOLATE AS FLUORESCENCE ENHANCING AGENT

LUCONI, MARTA; HENESTROSA, CECILIA; ACOSTA, GIMENA; PERALTA, CECILIA; FERNANDEZ, LILIANA

Rosario

Congreso; 4ta. Reunión Internacional de Ciencias Farmacéuticas; 2016

RiCiFa

Carbamazepine (CBZ) is a drug widely used as anticonvulsivant agent. It also has been used for trigeminal neuralgia and schizophrenia treatments. Additionally, CBZ is included in the group of emerging contaminants being one of the most frequently detected pharmaceutical in sewage-treatment plant effluents, river water and drinking [1]. CBZ is not fluorescent in solution but can be converted into a fluorescent compound using photo-induced fluorescence which is based on the photochemical transformation by using UV irradiation [2].The aim of this work is the development of a strategy of CBZphotodegradationin alkaline medium, without involving organic solvents, employing the bile salt Taurodeoxycholate (TDC) as enhancing fluorescent signal agent.A Shimadzu RF-5301PC spectrofluorimeter (Kyoto, Japan), equipped with a Xenon discharge lamp was used for the fluorescent measurements. A 1.0 cm quartz cell was employed using λex=251nm and λem=434nm, (slit widths 3/3). A home-made continuous photochemical reactor was constructed by a Minipuls peristaltic pumps from Gilson (Villiers, France) and coiling PTFE tubing (i.d. = 0.8 mm and o.d. = 1.0 mm) around an Hg vapor lamp (15W G15T8 UV-C, PHILIPS) and covered by an aluminum foil to permit the maximum reflectance of UV light. The influence of different agents on the fluorescent properties of CBZ photoproduct´s was assayed (synthetic and natural tensoactives, rhodamine B, etc.) and the pH reaction media was optimized. Moreover, the experimental parameters related with photodegradation was studied, i.e. reactor coil long (50-100 cm), irradiation flow rate (2.5-5.0 rpm). The best results were obtained in alkaline media (pH 11), using 200 l TDC (2x10-3mol L-1) as enhancing fluorescent signal agent, obtaining LOD = 1.7x10-7 molL-1, LOQ = 5.1x10-7 molL-1 and a calibration curve I= 6.0x107C (r2 = 0.9955) under the optimal conditions. This methodology will be automated and applied to the analysis of pharmaceutical and environmentalsamples.