SEQUENTIAL DETERMINATION OF NICKEL AND CADMIUM IN TOBACCO AND MOLASSES SAMPLES BY MOLECULAR FLUORESCENCE

ALESSO, MAGDALENA; FERNANDEZ, LILIANA; TALÍO, MARÍA CAROLINA

Mendoza

Congreso; XXXIV Reunión Anual de la Sociedad de Biología de Cuyo; 2016

Smoking cigarettes is harmful for health. In last time, alternatives like electronics cigarettes and narguille or water pipe (WP) have appeared. However, security is no proven. In present research, a new methodology for sequential determination ofNi(II) and Cd(II) is proposed and applied to tobacco and snuff used in WP (molasses). In a first step, a chemofiltration on nylon membrane is carried out employing eosine (Eo) and carbon nanotubes dispersed in SDS solution (phosphate buffer pH 7). In this conditions, Ni(II) was selectively retained on the solid support. Filtrate liquid was re- aconditioned with acetic acid /acetate (pH 5) to Cd (II) determination. Experimental parameters that influence efficiency of separative and determinative step were studied and optimized, like nature of membrane, nature and concentration of fluorophore, pH and buffer concentration. Working at optimal conditions, quantitative recoveries > 99.9%) were reached for both analytes with detection limits of ug L-1. Calibration of the new methodology presents a linearity of magnitude three-orders. The developed methodology was successfully applied to determination of Ni(II) and Cd(II) in molasses and conventional tobacco samples. The found concentration of both metals enables us to ensure that the use of water pipe is as harmful to health as smoking cigarettes.