EVALUATION OF MICRONUCLEI FREQUENCY INDUCED BY Aristolochia argentina IN MOUSE BONE MARROW

GARCIA J.; PEREIRA S.; SOSA A.; FUSCO M.; WENDEL G.; PELZER LE.

Mendoza-Argentina

Congreso; XXVI Annual Scientific Meeting of Cuyo Biology Society; 2008

68. EVALUATION OF MICRONUCLEI FREQUENCY INDUCED BY Aristolochia argentina IN MOUSE BONE MARROW García J., Pereira S., Sosa A.,  Fusco M., Wendel G., Pe!zer LEI. ´Farmacología. !Farmacognosia, Fac Qca, Bioqca y Fcia. Univ Nac San Luis, 5700 San Luis. E-mail: gwendel@unsl.edu.ar Aristolochia argentina (A. a.) (family Aristolochiaceae), popularly known as "charrúa". is used in folk medicine as: diuretic, antidiarrheic. astringent and antihemorroidal. The acute toxicity test after ora administration of 2000 mg/kg of  A.a revealed non­toxicity at this dose. This study examined the genotoxicity of. A.a  using micronucleated polychromatic erythrocytcs (MN-PCE) in bone marrow, of Rockland mice. Animals (3 males and 3 females) were injected intraperitoneally with destilled water (control group). A.a. 250 and 500 mg/kg and paraeetamol 1 g/kg (positive control). Mice were killed at 24 01´ 48 h after dosing. Bone marrow, cells were smeared, fixed with methanol, and stained with May Grunwald Giemsa. The presence of MN was evaluated in 2000 PCE for each animal. Our results showed that there were no differences in MN frequency per 1000 PCE (mean ± SEM) between A.a.-treated groups (250,500 mg/kg: 5.83 ± 0.86,5.41 ± 1.35, respectively) and con­trol group (4.33 ± 0.30) at 24 h. This effect was also observed at 48 h (A.a. 250,500 mg/kg: 5.66 ± 1.04.4.75 ± 0.77. respectivdy vs. control group: 4.16 ± 0.30). Administration of paracetamol pro­duced a significant increase (p<0.000 1) in MN frequcncy at 24 h (13.83 ± 0.(2) and at 48 h (15.00 ± 0.46) compared with that con­trols. These data indicate that in vivo A.a. has neither aneugcnic nor clastogenic effects.