NUEVA METODOLOGÍA PARA LA DETERMINACIÓN DE ALUMINIO EN MUESTRAS DE ORINA MEDIANTE FLUORESCENCIA EN FASE SÓLIDA.

FERNANDEZ LILIANA PATRICIA; SANTAROSSA DEBORA GIMENA; TALIO MARÍA CAROLINA

MENDOZA

Congreso; XXXIV Reunión Cientifica Anual de la Sociedad de Biología de Cuyo; 2016

NEW METHODOLOGY FOR DETERMINATION OF ALUMINIUM IN URINE SAMPLES BY SOLID SURFACE FLUORESECENCESantarossa, DG2, TalioMC2, ,Fernández LP12, 1Área de Química Analítica, Facultad de Química, Bioquímica y Farmacia, UNSL, 2 Instituto de Química de San Luis (INQUISAL-CONICET), Chacabuco y Pedernera, 5700 San Luis, Argentina. E-mail: magdalesso@yahoo.com.arAluminum (Al) is a toxic metal, capable of interfering with a variety of cellular and metabolic processes of the nervous system and tissues of the organism. Al has been considered as a possible cause of renal osteodystrophy, Parkinson's and Alzheimer's disease. In this work, the separation/preconcentration of Al(III) on nylon membrane (0.45 µm pore size) is carried out employing quinizarina 1x10-5 mol L-1 and SDS micellar solution 2x10-2 mol L-1 (acetic/acetate buffer pH 5) for later quantification by solid surface fluorescence (λexc = 490 nm; λem = 570 nm; slits = 1.5/3). Experimental parameters that influence efficiency of separative and determinative step were studied and optimized (nature of membrane, nature and concentration of fluorophore, pH and buffer concentration). Working at optimal conditions, quantitative recovery > 99.9% of Al (III) was reached with detection limits of g L-1. Calibration of the new methodology presents a linearity of three-orders of magnitude. The developed methodology was successfully applied to determination of Al(III) in urine samples. The proposed methodology represents an alternative to the routine metal analysis methods, with the advantage of using a simple inexpensive instrumental as a spectrofluorometer.Farmacología y Toxicología (FT)- Provincia de San Luis.