Monitoring oxidative stress parameters as chlorophyll level in cadmiun treated Glycinemax L. plants by molecular fluuorescence

FELICI CE; WANG CC; ZIRULNIK F; GÓMEZ MR; FERNÁNDEZ, LP

Estancia Grande, San Luis

Congreso; encuentro anual de sociedad de biología de cuyo; 2015

Sociedad de Biología de Cuyo

Heavy metals induce different types of stress in plants. The monitoring of chlorophyll a (chla) level is a good indicator ofan imbalance in the redox homeostasis and therefore of oxidative stress. The aim of this work was to determine thedecrease of chlorophyll level that reveal the oxidative stress caused for Cd exposure. Soybean seeds were germinated anddeveloped under controlled conditions until were used. On the 4th day of germination were placed in hydroponicconditions with Hoagland nutrient solution. On the 10th day, were exposed to Cd (40 μM) during 4, 6, 24, 72 and 144 h.To obtain extracts, 500 mg of leaves were homogenized under ice-cold conditions in ethanol absolute. Homogenates werecentrifuged for 10 min and the supernatants were analyzed. To carry out a methodological comparison, the chladetermination was measured by a-spectrophotometry and b-fluorescence. The reduction in chlacontent measured byspectrophotometry was significantly higher at 24, 72 and 144 h in Cd-treated plant (*p