Effect of MWCNT as SPE packing materialforthe separationof chiralemerging contaminants

LANARO VERÓNICA M.; PERALTA CECILIA M.; SOMBRA LORENA L.; STEGE PATRICIA W.

Córdoba

Congreso; III Reunión internacional de ciencias farmaceuticas; 2014

Nowadays the emerging pollutants have a great concern, and between them the most studied are pharmaceutical drugs. Thesechiral compound often exhibit different effects in pharmacological activity, transport mechanism, metabolism pathway and toxicity.Specifically,β-blockers are soldandadministered asaracemic mixture, butthe (S) enantiomeris pharmacologically active one. Paradoxically, the (R)enantiomeris the one which hasnegativeand toxic effects.Carboxylatedmultiwalledcarbonnanotubes (cMWCNT)have shown interesting characteristics as enantioselectivepacking or stationary phase material in separative methods. The aim of this work was develop a method able to separate a racemic mixture of emerging pollutants. The studied analytes were β-blockers (pindolol, propranolol and labetalol) commonly used in the treatment of heart rhythm disorders. We performed a separation procedure usingcarboxylatedandnon-carboxylatedmultiwalledcarbonnanotubes (ncMWCNT)asSPE packing material. For theassembly of the columns,2.7 to12 mg of MWCNT was weighed and the elution solution was water. On the back of the column a 0.45 μfilter wasplaced to retain MWCNT. Theanalyteswere determinedusing anHPLC system with a C18 column andthemobile phase usedwasAcetinitrilo/NH4HCO3(50:50), pH 10 with a PDA detector. The results showed that using ncMWCNT there were not separation for any of the studied analytes. In the case of cMWCNT the results for the three studied analytes were different. In the case of labetalol there was not separation, however for propranolol the separation was not so evident, and the result may be improved with some simple changes in the eluent. But, for pindolol the separation was very clear. The preliminary conclusions of the work were that cMWCNT could be an interesting and economic alternative for the separation of chiral drugs. The next step is the molecular study of the interaction the three drugs with the cMWCNT.