MONITORING OXIDATIVE STRESS PARAMETERS AS CHLOROPHYLL LEVEL IN CADMIUN TREATED Glycine max L. PLANTS BY MOLECULAR FLUORESCENCE

FELICI, EMILIANO; WANG, CHIEN CHUN; ZIRULNIK, F.; GOMEZ, ROXANA A.; FERNANDEZ, LILIANA

San Luis

Congreso; XXXI Reunión Anual de la Sociedad de Biología de Cuyo; 2014

Sociedad de Biología de Cuyo

Heavy metals induce different types of stress in plants. The monitoring of chlorophyll a (chla) level is a good indicator of an imbalance in the redox homeostasis and therefore of oxidative stress. The aim of this work was to determine the decrease of chlorophyll level that reveal the oxidative stress caused for Cd exposure. Soybean seeds were germinated and developed under controlled conditions until were used. On the 4th day of germination were placed in hydroponic conditions with Hoagland nutrient solution. On the 10th day, were exposed to Cd (40 µM) during 4, 6, 24, 72 and 144 h. To obtain extracts, 500 mg of leaves were homogenized under ice-cold conditions in ethanol absolute. Homogenates were centrifuged for 10 min and the supernatants were analyzed. To carry out a methodological comparison, the chla determination was measured by a-spectrophotometry and b-fluorescence. The reduction in chla content measured by spectrophotometry was significantly higher at 24, 72 and 144 h in Cd-treated plant (*p