OXIDATIVE STRESS PARAMETERS MONITORING IN CADMIUN TREATED GLYCINE MAX L. PLANTS

FELICI, EMILIANO; MOLINA, ALICIA; ARCE, SILVIA; GIL, RAUL; ACOSTA, GIMENA; ZIRULNIK, FANNY; GOMEZ, MARÍA ROXANA

San Luis

Congreso; XXX Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2012

Sociedad de Biología de Cuyo

Heavy metals induce different types of stress in plants. The monitoring of essential elements concentration, the analysis of chlorophyll level and the study of ionic permeability are good indicators of oxidative damage in the plant homeostasis. The aim of this work was the study of the parameters that reveal the oxidative stress caused for Cd exposure. Soybean seeds were germinated and developed under controlled conditions were used. On the 4th day of germination were placed in hydroponic conditions with Hoagland nutrient solution. On the 10th day, were exposed to Cd (40 µM) during 4, 6, 24, 72 h and 6 days. The seedlings were harvested and analyzed after microwave assisted digestion. The concentration of Cd, Cu, Fe, Mg, Zn and Mn in soybean roots and leaves was obtained by ICP-MS (Inductively coupled plasma-mass spectrometry). High levels of these elements were found in Cd treated plants. Chlorophyll level and ionic permeability were determined. At 24 h a reduction in the chlorophyll level was observed, indicating chloroplast damage. This parameter is related with a significant decrease in Mg uptake at leaf level. Ionic permeability was modified in Cd treated plants. Cu, Fe, Zn and Mn increased due to Cd exposure, it could be related with the antioxidant enzymes which use these elements as cofactors (CuZnSOD, FeSOD, MnSOD). In this model, Cd exposure alters the normal physiological status of soybean plants.