CHLORPHENIRAMINE MALEATE ENANTIOSEPARATION USING CARBON NANOTUBES AND CYCLODEXTRINS AS CHIRAL SELECTORS BY FIA WITH FLUORESCENT DETECTION

ACOSTA, GIMENA; RAUL SILVA; RAUL A. GIL; ROXANA, GOMEZ; LILIANA P. FERNÁNDEZ

Rosario

Congreso; Reunión Internacional de Ciencias Farmacéuticas RICiFa; 2012

Universidad Nacional de Rosario

Many drugs used in therapeutics have a chiral center and in most cases the pharmacological activity is restricted to one of the enantiomers; whereas the remaining form show no effect or produce different therapeutic. Chlorpheniramine maleate (CPA) is used in the treatment of allergic, to relieve rhinitis, sneezing, itchy eyes, nose and throat. The International Conference on Harmonization (ICH) establish, for the quality control of chiral drugs marketed as single enantiomer, that the undesired enantiomer must be considered as any other impurity (1). Hence, the development of methods for enantioselective analysis which enable monitoring of the enantiomeric purity becomes very important tools for drug quality control. The multiwalled carbon nanotubes (MWCNTs) have been applied for the development of higher performance separation techniques that utilize nanoscale interactions (2). Moreover, considering their chiral structure, MWCNTs have been applied in order to achieve enantioseparation of pharmaceutical compounds. On the other hand, the use of cyclodextrins (CDs) as chiral selectors is the most frequently approach applied in the enantiomer separations. A multicriterium optimization was applied to find the best conditions that met the analytical requirements (3). The aim of this work was to develop a methodology of flow injection-solid phase extraction (FI-SPE) with fluorescence detection for the enantioseparation of (±)-CPA using MWCNTs and β-CD.