ELECTROCHEMICAL DETECTION OF ZINTEROL IN SERUM BOVINE SAMPLES IN A SCREEN PRINTED CARBON ELECTRODE MODIFIED WITH METAL-OXIDE CUPPER NANOPARTICLES

REGIART D. M; BERTOLINO FRANCO A.; MARTINEZ N. A.; DEBÁN L; VÁZQUEZ MD; JULIO RABA

Congreso; “2ª Reunión Internacional de Ciencias Farmacéuticas” (RICiFA); 2012

Introduction Zinterol (Zin) is a β-agonist, which is used in the symptomatic treatment of asthma, chronic bronchitis and other allergic diseases associated with respiratory pathway. However β-agonists have been used as growth promoters in the livestock production, because it promotes muscle growth and lipid degradation. Moreover, due to its long half-life and stability, the residues could present a potential risk for human health, because can cause symptoms of acute poisoning in human (1). Symptoms from β-agonists residue-induced food poisoning have been reported from investigations of separate events in several countries. Moreover, drug residues may negatively impact the export trade of edible animal products and result in nearly incalculable economic loss. Therefore, the use of such drugs as animal feed additives has been banned in most countries (2). In the present work we proposed the incorporation of metal-oxide cupper nanoparticles in the surface of a screen printed carbon electrode (SPCE) to improve the detection limit in the electrochemical determination of Zin. The SPCE offer many potential advantages including reduced reagent consumption, smaller analysis volumes, faster analysis times, and increased instrument portability (3). Moreover, we used a β-agonist antibody which was previously immobilized in an amino functionalized column (AFC) to eliminate interference from the sample as well as to pre-concentrate the drug. The CuNPs-CuONPs were characterized by transmission electron microscopy (TEM) and cyclic voltammetry (CV). This method has many characteristics such as high sensitivity, fast response time, wide linear range, good selectivity, stability, and reproducibility.