Optimization of a capillary electrophoresis methodology for the simultaneous determination of veterinary antibiotic drugs

CASADO, CRISTIAN; QUINZIO, EUGENIA; CAÑAS, IRENE; GOMEZ, MARÍA ROXANA; NASSETTA, MIRTHA

Simposio; 18th Latin American Symposium on Biotechnology, Biomedical, Biopharmaceutical and Industrial Applications of Capillary Electrophoresis and Microchip Technology; 2012

Antibiotics are widely used in agriculture as growth enhancers and for disease treatment and control in animal feeding operations. In the north of the province of Cordoba, has increased the use of avermectins and milbemicins in veterinary medicine for the treatment of parasitic infections in goats. This causes impact both in soils and in water due to elimination of the drugs in the feces of the treated animals. Thus, monitoring the presence of these compounds and other veterinary drugs in surface water is of particular concern to public health in general. An accurate assessment of their occurrence depends on development of sensitive and selective analytical methods based on modern technologies. The aim of this work was to study the influence of experimental conditions on the separation and determination of Ivermectin (IVM) and Moxidectin (MXD) by capillary electrophoresis (CE). Considering the nature of the analytes, MEKC and MEEKC separation modes were investigated. The best results were obtained by using a microemulsion background electrolyte (BGE) containing a 10 mM borate buffer at pH 9.2, 0.05% (v/v) heptane (10 mM) as oil droplets, 40 mM sodium dodecylsulphate as surfactant, 930.6 mM 1-butanol as co-surfactant and 3 % (v/v) ethanol as organic co-solvent. The investigated analytes were separated y determined in less than 10 minutes with good accuracy and precision. The developed method can be applied to monitoring the environmental occurrence of IVM and MXD in water in a simple, fast and reliable way. Also, more experience is necessary to adapt the methodology to the analysis of these analytes in milk and related products.