Sensitive preconcentration coupled to simple chromatographic method for local anesthetics monitoring in environmental waste water samples

GIMENA ACOSTA; MARIA ROXANA GOMEZ; MANUEL SOLARI ; CHIEN CHUNG WANG; ANA VICARIO ; MARCELA LONGHI

Cordoba

Congreso; 6ta Reunión Internacional de Ciencias Farmacéuticas (RICiFa 2020+1); 2021

Abstract: Local anesthetics (LA) are a group of compounds used in medical practice to treat or relieve painful conditions. Our purpose was to achieve the simultaneous and sensitive determination of five LAs: Benzocaine (BNZ), Bupivacaine (BPV), Carticaine (CAR), Prilocaine (PRI) and Procaine (PRO) in wastewater. An off-line separation and preconcentration method, using a XAD-4 resin column followed by HPLC/UV quantification at λ = 232 nm, was developed for the analysis of LAs in environmental samples. The experimental design, data analysis and desirability function calculations for the quantification were performed by using Stat-Ease Design-Expert Trial Version 8.0 software. From the initial 100 ml sample, 5 ml eluates were obtained, with no less than 95% of mass recovery for each analyte, improving 19 times the methodology sensitivity. The observed HPLC retention times of BPV, CAR, PRI, PRO and BNZ were 0.88, 1.99, 6.17, 6.92 and 12.27 min respectively. The limits of detection (LOD) and limits of quantification (LOQ) of the studied LAs were between 2.47 10-7-7.46 10-7 (µg ml-1) and between 8.06 10-7-2.49 10-6 (µg ml-1), respectively. In all cases, the standard deviation obtained was lower than 0.05 for both intraday and interday precision. In conclusion, the sensitivity and selectivity of the proposed method offers a tool for simultaneous screening and quantification of all the local anesthetics studied.