IFEC   20925
INSTITUTO DE FARMACOLOGIA EXPERIMENTAL DE CORDOBA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Activation of catalase by 3-nitropropionic acid increases voluntary ethanol intake in control rats as compared to perinatally Pb-exposed animals
Autor/es:
MATTALLONI M.S., CANCELA L., VIRGOLINI MB.
Lugar:
Mar del Plata
Reunión:
Congreso; LV Reunión Anual de la Sociedad Argentina de Investigación Clínica. Reunión de la Sociedad Argentina de Fisiología. XLII Reunión Annual de la Sociedad Argentina de Farmacología Experimental.; 2010
Institución organizadora:
SAFE. SAIC
Resumen:
ACTIVATION OF CATALASE BY
3-NITROPROPIONIC ACID INCREASES VOLUNTARY ETHANOL INTAKE IN CONTROL RATS AS
COMPARED TO PERINATALLY Pb-EXPOSED ANIMALS
Mara S. Mattalloni, Liliana
M. Cancela and Miriam B. Virgolini
IFEC-CONICET. Depto de
Farmacologia. Facultad de Ciencias Químicas. Universidad Nacional de Córdoba. Córdoba.
Argentina
Keywords: catalase- lead exposure- ethanol- 3-nitropropionic acid- 3-amino
1,2,4 triazole
Catalase (CAT) is an antioxidant enzyme with a key role in brain
ethanol (ET) metabolism. We have demonstrated that the chronic administration
of a CAT inhibitor, 3-amino 1,2,4 triazole (AT) prevents the manifestation of
the increased voluntary ET intake and blunted the elevated CAT activity in
blood and several brain regions from Pb-exposed animals. In the present study
we sought to determinate the effects of the over activation of CAT on ET intake
in both, control and Pb-exposed rats. Thirty-five day-old male Wistar rats
exposed to 220 ppm Pb during gestation and lactation were submitted to voluntary
ET intake during 2 h/day for 28 days. Once stable 10% ET intake was achieved, a
subset of animals was injected with vehicle (SAL) or 30 mg/kg of 3-nitropropionic
acid (3-NPA), a neurotoxin that boosts CAT activity (days 25-28; groups: 63d-ET-SAL
and 63d-ET-chronic3NPA, respectively) while another subset of animals was
injected with a single dose of 3-NPA (day 28; group 63d-ET-acute3NPA). All rats
were sacrificed on day 28 immediately after the last free-choice ET session, and
brain regions harvested to measure brain CAT activity. Blood was collected to analyze
CAT activity, Pb, and ET levels. The results demonstrated that chronic (but not
acute) 3-NPA administration increased voluntary ET intake in control animals to
levels comparable to those of the Pb-exposed group injected with SAL or 3-NPA.
The failure of the CAT activator to further increase ET intake in the Pb-exposed
rats may be due to a ceiling effect or to 3-NPA neurotoxic effects on striatal
neurons. The increases in blood CAT activity in response to 3-NPA were similar in
both groups while the NAc was the only brain region from the Pb-exposed group that
did not show an increase in brain CAT activity in response to chronic 3-NPA
administration. These results are discussed in terms of the putative role of
CAT in the increased voluntary ET intake observed in Pb-exposed rats.
Sources: SeCyT- CONICET- FONCyT