G-protein participates in the chitosan–induced Anthraquinone production in R. tintorum l

VASCONSUELO, A;; PICOTTO, G;; GIULIETTI, AM; BOLAND R.

Misiones

Congreso; XL Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular - SAIB.; 2004

 Plants have acquired economical importance due to the synthesis of secondary metabolites, but the levels produced in vitro are generally too low for commercial applications. Elicitation with chitosan (200 mg/l) significantly stimulated (@100%) anthraquinone (Aq) synthesis in R. tinctorum cultures. Studies have involved in the chitosan action the activation of the phospholipase C (PLC)/protein kinase C (PKC) pathway, phosphoinositide 3’-OH-kinase (PI3K), mitogenic activated protein kinase (MAPK) cascade and the participacion of Ca2+. Here demonstrated  that the elicitation  could be blocked with 1 mM suramin, 1 mM GDPßS or with 20 mg/ml of anti-Gαq/11 antibody. In addition chitosan induction  could be mimic  by 10 mM mastoparan, 10 mM  AlF-4 or with 300 mM GTPgS. These evidences strongly indicate that a G-protein, with immunological characteristic  of Gαq/11,   is the nexus between extracellular chitosan’s signal  and the intracellular messengers systems above mentioned.