Molecular imprinting applied to the analysis of nanoencapsuled Coenzyme Q10

GARCIA BECERRA C.; CONTIN M.; MORETTON M.; LUCANGIOLI S.; CHIAPPETTA D.; TRIPODI V.

Cordoba

Jornada; 3ª Reunión Internacional de Ciencias Farmacéuticas; 2014

RICIFA

Coenzyme Q10 (CoQ10) is an essential cofactor in mitochondrial oxidative phosphorylation and a powerful antioxidant. It is used as a therapeutic agent in certain conditions like cancer, Parkinson, Alzheimer, cardiovascular, mitochondrial, neurological and muscular diseases. However, due to its high hydrophobicity and low permeability, CoQ10 bioavailbility is extremely reduced. That is why it was decided to employ a polymer-based nanoparticle approach to improve it. CoQ10 is currently analyzed by HPLC in pharmaceutical and biological matrices. In our experience, HPLC analysis of nanoencapsulated drugs, implies an intrinsic challenge due to the potential presence of chromatographic interferences and reduced lifetime in the chromatographic column produced by the polymer and surfactants which are dissolved with the drug in the sample.In order to minimize these problems, it was decided to apply a clean up technique based on a molecularly imprinted solid phase extraction (MISPE), previously developed by this group, which have demonstrated a high specificity for CoQ10. The MISPE extract was analysed by a micro-HPLC system developed by our group.