Miniaturized imprinted solid phase extraction to the selective analysis of Coenzyme Q10 in urine

GARCIA BECERRA C.; BAEZ F.; LUCANGIOLI S.; FLOR S.; TRIPODI V.

JOURNAL OF CHROMATOGRAPHY B

ELSEVIER

Lugar: Amsterdam; Año: 2019 vol. 1116 p. 24 - 28

0378-4347

Coenzyme Q10 (CoQ10) is an important cofactor in the mitochondrial respiratory chain and a potentendogenous antioxidant. CoQ10 deficiency is currently associated with numerous diseases likemitochondrial and neurodegenerative pathologies, in which the earliest diagnosis and treatment withCoQ10 supplementation becomes paramount for patient?s treatment. Consequently, the determination ofCoQ10 levels in different biological matrices positions as a fundamental tool. Urine is an attractive andnon-invasive alternative source to tissue, blood or other biofluids for CoQ10 analysis. However, it posesan analytical challenge, as it generally requires a complex sample preparation, with multiple steps. In thiswork we developed and validated a molecularly imprinted polymer solid phase extraction (MIP-SPE)followed by an HPLC-MS/MS method for the analysis of CoQ10 in urine. The MIP-SPE methoddeveloped is simple and fast compared to previously traditional reported methods, with reducedprocessing time, improved sample cleaning and excellent recovery values, along with its inherent highselectivity. The developed chromatographic method was validated according to FDA guidelines, anddemonstrated to be suitable for the analysis of CoQ10 in urine samples with LOQ and LOD values of 0.6ng/mL and 0.2 ng/mL of CoQ10 in urine respectively. Recovery values at three concentration levels werehigher than 90.0 %.The proposed method is amenable to be applied in pediatric patients due to the lowsample requirement and useful for diagnosis and post-treatment control