Development and validation of a CD-MEKC system for the simultaneous determination of dihydrostreptomucin sulfate and two benzylpenicillin salts

FLOR S.; IGLESIAS RANDO M.; CONTIN M.; SCIOSCIA S.,; TRIPODI V.,; LUCANGIOLI S.

JOURNAL OF LIQUID CHROMATOGRAPHY AND RELATED TECHNOLOGIES

TAYLOR & FRANCIS INC

Lugar: Londres; Año: 2015 vol. 38 p. 955 - 962

1082-6076

A CD-MEKC system using sodium dodecyl sulfate and sulfated--cyclodextrin to achieve the 15 simultaneous separation of Penicillin G procaine, Penicillin G benzatine and dyhidrostreptomycin sulfate 16 in pharmaceutical and veterinary dosage forms, was developed. Several parameters were evaluated, 17 including buffer type and concentration, cyclodextrin type and concentration, organic solvent and 18 instrumental parameters. The optimized system consisted of 50 mM SDS, 1% sulfated-ß-cyclodextrin 19 and 10 mM pH 9.0 borate buffer. Capillary temperature was kept at 35ºC, and electrophoretic system 20 was operated under positive polarity at a constant voltage of 18 kV. 21 Validation parameters such as specificity, linearity, LOD and LOQ, precision, accuracy and robustness 22 were assayed. Therefore, the developed method was found to be appropriate for quality control of 23 Penicillin G procaine, Penicillin G benzatine and dyhidrostreptomycin sulfate.