INVESTIGADORES
LUCANGIOLI Silvia Edith
artículos
Título:
A novel non-invasive sampling method using buccal mucosa cells for determination of coenzyme Q10
Autor/es:
MARTINEFSKI M.,; SAMASSA P; LUCANGIOLI S.,; TRIPODI V.
Revista:
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Editorial:
SPRINGER HEIDELBERG
Referencias:
Lugar: Heidelberg; Año: 2015 vol. 407 p. 5529 - 5533
ISSN:
1618-2642
Resumen:
Abstract Coenzyme Q10 (CoQ10) is an important cofactor in the mitochondrial respiratory chain and a potent endogenous antioxidant. CoQ10 deficiency is often associated with numerousdiseases,and patients canbenefit fromCoQ10 supplementation,being moreeffectivewhendiagnosed and treated early. Due to the increased interest in CoQ10 deficiency, several methods for CoQ10 analysis from plasmatic, muscular, fibroblast, and platelet matrices have been developed. These sampling techniques are not only highly invasive but alsotootraumaticforperiodicclinicalmonitoring.Inthepresent work, we describe the development and validation of a novel non-invasive sampling method for quantification of CoQ10 in buccal mucosa cells (BMCs) by microHPLC. This method is suitable for using in a routine laboratory and useful forsamplingpatientsinpediatry.CoQ10correlationwasdemonstrated between BMCs and plasma levels (Spearman r, 0.4540; p
