INVESTIGADORES
LUCANGIOLI Silvia Edith
artículos
Título:
Spectroscopic approach of the association of heparin and its contaminant andrelated polysaccharides with polymers used in electrokinetic chromatography
Autor/es:
FLOR S; TRIPODI V.; CONTIN M.; DOBRECKY C.,; LUCANGIOLI S.
Revista:
Journal of Chemical and Pharmaceutical Research
Editorial:
Journal of Chemical and Pharmaceutical Research
Referencias:
Año: 2012 vol. 4 p. 972 - 979
ISSN:
0975-7384
Resumen:
Heparin (Hep) is a linear sulfated polysaccharide used in clinical treatment of tromboembolic diseases. In 2008,
contaminated batches of Hep appeared in the pharmaceutical market. The contaminant was identified as an over
sulfated form of chondroitin sulfate (oversulfated chondroitin sulfate, OSCS). Different analytical methods have
been developed to identify and quantify OSCS and other impurities expressed as dermatan sulfate from Hep. In this
sense, we have developed a capillary electrophoretic method based on the use of a mixture of polymers such as
polymeric b-cyclodextrin and poloxamine. As a result of this combination, not only the resolution of Hep from its
impurities and contaminant was obtained but the sensitivity was extremely improved. The association of Hep and its
related polysaccharides with the polymers was characterized by spectroscopic techniques as ultraviolet (UV) and
infrared (IR). The results suggest that the interaction between polysaccharides and polymers would principally be
through the sulfate groups of polysaccharidesb-cyclodextrin and poloxamine. As a result of this combination, not only the resolution of Hep from its
impurities and contaminant was obtained but the sensitivity was extremely improved. The association of Hep and its
related polysaccharides with the polymers was characterized by spectroscopic techniques as ultraviolet (UV) and
infrared (IR). The results suggest that the interaction between polysaccharides and polymers would principally be
through the sulfate groups of polysaccharides