Simultaneous determination of nine endogenous steroids in human urine by polymeric-mixed micelle capillary electrophoresis.

SABRINA FLOR; SILVIA LUCANGIOLI; MARIO CONTIN; VALERIA TRIPODI

ELECTROPHORESIS

WILEY-V C H VERLAG GMBH

Lugar: WEINHEIM; Año: 2010 vol. 31 p. 3305 - 3313

0173-0835

A new capillary electrophoresis system based on the use of polymeric-mixed micelles (cholic acid, SDS and the poloxamine Tetronic â 1107), was developed for the simultaneous determination of nine steroids in human urine. This method allows the baseline separation and quantitation of cortisol, androstenedione, estriol, dehydroepiandrosterone sulfate, testosterone, dehydroepiandrosterone, estrone, progesterone and estradiol in less than 25 minutes showing to be sensitive enough to detect low concentrations of these steroids in urine samples (5-45 ng/mL). The optimized electrophoretic conditions was performed using a 50 cm x 75 µm capillary, 18 kV, 25 ºC, with 44 mM CA, 10 mM SDS, 0.05% w/v tetronic Ò 1107, 2.5% v/v methanol, 2.5% v/v tetrahidrofuran in 5 mM borate- 5 mM phosphate buffer (pH=8.0) as a background electrolyte and a dual 210/254 UV-detection. The method can simultaneously determine 0.1-120µg/mL which corresponds to 5-6000 ng/mL of steroids in 2 mL urine. The recoveries ranged between 82.4 and 101.5%. Due to its simplicity, speed, accuracy and reliability, the proposed method could be a potential alternative to the traditional methodologies used with clinical purposes.