INVESTIGADORES
LUCANGIOLI Silvia Edith
artículos
Título:
A capillary electrophoretic system based on a novel microemulsion for the analysis of coenzyme Q10 in human plasma by electrokinetic chromatography
Autor/es:
LUCANGIOLI S.; FLOR S.; CONTIN M.; TRIPODI V.
Revista:
ELECTROPHORESIS
Editorial:
WILEY-V C H VERLAG GMBH
Referencias:
Año: 2009 vol. 30 p. 1899 - 1905
ISSN:
0173-0835
Resumen:
A new analytical method for determination of coenzyme Q10 (2,3-dimethoxy-5-methyl-6-
decaprenyl-1,4-benzoquinone, CoQ10) in human plasma was developed based on CE
using a double tensioactive microemulsion. CoQ10 was quantitatively extracted into
1-propanol/hexane and quantified by MEEKC. The microemulsion was prepared by
mixing 1.4% w/w sodium bis(2-ethylhexyl) sulfosuccinate, 4% w/w cholic acid, 1% w/w
octane, 8.5% w/w butanol, 0.1% w/w PVA and 85% w/w 10mM Tris buffer at pH 9.0.
The optimized electrophoretic conditions included the use of an uncoated silica capillary
of 60 cm total length and 75 mm id, an applied voltage of 20 kV, room temperature and
214 nm ultraviolet detection. Selectivity, linearity, LOD, LOQ, precision and accuracy
were evaluated as the parameters of validation. Owing to its simplicity and reliability, the
proposed method can be an advantageous alternative to the traditional methodology for
the quantitation of CoQ10 in human plasma with good accuracy and precision.mm id, an applied voltage of 20 kV, room temperature and
214 nm ultraviolet detection. Selectivity, linearity, LOD, LOQ, precision and accuracy
were evaluated as the parameters of validation. Owing to its simplicity and reliability, the
proposed method can be an advantageous alternative to the traditional methodology for
the quantitation of CoQ10 in human plasma with good accuracy and precision.