INVESTIGADORES
LUCANGIOLI Silvia Edith
artículos
Título:
ANALYSIS OF IMMUNOSUPPRESSIVE DRUGS AND THEIR PHARMACEUTICALS BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY
Autor/es:
TRIPODI V.,; LUCANGIOLI S.,; BARBARA C.,; RODRIGUEZ V.,; CARDUCCI C.
Revista:
CHROMATOGRAPHIA
Editorial:
FRIEDR VIEWEG & SOHN VERLAGSGESELLSCHFT
Referencias:
Lugar: WIESBADEN; Año: 2001 vol. 54 p. 93 - 98
ISSN:
0009-5893
Resumen:
A simple and practical micellar electrokinetic capillary chromatography (MEKC) method is proposed for the quantitation of immunosuppressive drugs such as azathioprine (AZ¡".), myco- phenolate mofetil (MMF), cyclosporine A (CyA) and tacrolimus (FK 506). The electrophoretic se- paration of the analytes was performed with a background electrolyte containing 20 mM phosphate buffer at pH 7.5, 50 mM SDS and methanol as an organic modifier. Fused silica ca- pillaries 75 ¡.tm i. d. and 60 cm in length were employed and detection of analytes was per- formed at 214 nm.Thorough validation according to international guidelines showed that the proposed meth- od is reliable and appropiate for the routine analysis of immunosuppressants. Moreover, it may be an advantageous alternative to the traditional chromatographic methodologies currently employed in the pharmaceutical and bioanalysis fields.
