Clostridum septicum AND Clostridium chauvoei INDUCE DIFFERENT TLRS EXPRESSION

GUERRA, RA; CÁCERES, CS; VILLA, MC; CORTIÑAS, TI; BERÓN, W.

Mar del Plata

Congreso; LI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2015

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB)

Toll-like receptors (TLRs) are the best characterized innate immune receptors. Upon ligand binding, TLRs activate a signaling pathway that enhances expression of immune factors such as cytokines, provoking inflammation and communication with the adaptive branch of immunity. Clostridium chauvoei and Clostridium septicum are Gram positive anaerobic flagellated pathogens, with 99% genetic similarity but different immunity. Both pathogens are causative agents of myonecrosis, which is a deadly disease affecting cattle, man and many warm-blooded animals.The aim of this work was determine TLRs expression induced by C. chauvoei and C. septicum in peritoneal mouse macrophage. Macrophage (1 x 106 cell/well) were co-cultured with C. chauvoei and C. septicum cells obtained in log phase culture at different multiplicity of infection (MOI) for 4, 12 and 24h. RNA was reverse transcribed into cDNA and used for RNA amplification with specific primers, using RT-PCR. The bands were analyzed by Scion image software. The results showed that C. chauvoei cells induces 1.4-2 times increase of TLR 5 expression early in time,even at low MOI; while C. septicum induced TLR 2 expression, 2 times higher than that of control at 4 h postinfection. In conclusion, specific TLR2 would be singled out as a dominant receptor for C. septicum and TLR5 as a dominant receptor for C. chauvoei.