Expression of NHERF1 in Colonic Tumors Induced by 1,2-dimethylhydrazine in Rats is Independent of Plasma Ovarian Steroids

TRONCOSO M; CUELLO-CARRIóN; GUIñAZú E; FANELLI M.A.; MONTT-GUEVARA M; CABRINI R; CARON R; KREIMANN EL

Hormones and cancer

Springer

Año: 2011 vol. 2 p. 214 - 223

1868-8497

In normal embryonic fibroblasts, the Na+/H+ exchanger regulator factor 1 (NHERF1) stabilizes Ecadherin/+/H+ exchanger regulator factor 1 (NHERF1) stabilizes Ecadherin/ â-catenin binding and the lack of NHERF1 expression promotes cell transformation thus acting as a tumor suppressor gene. We here tested the hypothesis that NHERF1 could act as a tumor suppressor gene in colon cancer as a mediator of estrogens? protective actions in colon carcinogenesis. We studied the expression and localization of NHERF1 and â-catenin by immunohistochemistry in colonic tumors induced by 1,2 dimethylhidrazine (DMH) in Sprague? Dawley rats. One group of the rats treated with the carcinogen was ovariectomized (OVX) in the middle of the tumor induction, simulating a human menopausal condition. We observed a protective role of estrogens in colon cancer, as non-ovariectomized rats (DMH) had a reduced tumor area compared with the ovariectomized group (DMH+OVX; mean±SE) 28.98±4.65 vs. 67.58±8.69 (p