Brucella abortus-infected platelets activate the endothelium and promote the migration of monocytes towards the site of infection.

TROTTA A; MILILLO MA; RODRIGUEZ AM; BALBOA L; DELPINO MV; GIAMBARTOLOMEI GH; BARRIONUEVO P.

Mar del Plata

Congreso; LXVI Reunión Anual de la Sociedad Argentina de Inmunología (SAI); 2018

Sociedad Argentina de Inmunología (SAI)

Brucellosis is an infectious disease elicited by bacteria of the genus Brucella. Platelets have recently got involved in the modulation of innate and adaptive immune responses. We have previously reported that platelets act as carriers of bacteria, promoting the invasion of monocytes. Thus, the aim of this study was to further investigate the role of platelets in the immune response against Brucella. First, we wondered whether the presence of platelets modulate the time course of B. abortus infection. For this, THP-1 cells (human monocytic cell line) were infected with B. abortus in presence or absence of platelets. Then, extracellular bacteria were killed and cells were incubated for different times. Our results demonstrate that the presence of platelets significantly increased the percentage of B. abortus-infected THP-1 cells at early time-points (p