INVESTIGADORES
BARRIONUEVO Paula
congresos y reuniones científicas
Título:
HLA-B35 alleles in a tribe of Chiriguano indians from the North of Argentina.
Autor/es:
THEILER, GRACIELA; LINDEL, NANCY; SCHETTINI, JORGE; CAPUCCHIO, MÓNICA; BARRIONUEVO, PAULA; SATZ, LEONARDO
Lugar:
San Diego, California.
Reunión:
Otro; 22nd Annual Meeting.; 1996
Institución organizadora:
American Society for Histocompatibility and Immunogenetics.
Resumen:
HLA-B35 is a class I antigen that comprises at least sixteen alleles which differ among them by one to six amino acids. In the present work a rapid DNA typing procedure was used to investigate the HLA-B35 alleles in 14 individuals from a tribe of Chiriguano Indians where this serological specifity is present in 25% of the population. The approach is based on a group-specific PCR amplification of a sot of closely related HLA-B alleles sharing a Thr in position 45 of the a1-domain. The amplified DNA was then hybridized to a panel of sequence-specific oligonucleotide probes designed to recognize the polymorphic residues in previously reported HLA-B35 subtypes. The following B35 alleles were found: HLA-B35 allele: B*3501 (No. of individuals: 4*), B*3503 (No. of individuals: 1*), B*3505 (No. of individuals: 2), B*35v# (No. of individuals: 9*). * Individuals carring two different B35 subtypes. # New B35 alleles. Since this population has an estimated ethnic admixture of 10%, it can not be ruled out that the presence of N*3501 is of Caucasian origin. However, B*3501 has also been described in an isolated Cayapa population from Ecuador. B*3503 has also a wide distribution in different population and its presence in these individuals could be of Caucasian origin. B*3505 was originally described in Amerindians from the south of Brasil and therefore is not surprissing its presence among the Chiriguanos. Nine individuals had patterns of hybridization suggestive of at least two new B35 alleles, with replacements in residues 94, 95, 97 and 114 of the a2-domain. These variants are now being characterized by DNA sequencing. The results confirm the high degree of polimorphism of the HLA-B locus and specially HLA-B35 among the Amerindians.
