HLA-B35 alleles in 440 individuals from 16 different populations as assessed by DNA typing.

SATZ, LEONARDO; THEILER, GRACIELA; BARRIONUEVO, PAULA; SCHETTINI, JORGE; VILCHES, C; BETUEL, H; GEBUHRER, L; BRAUTBAR, C; PAPASTERIADES, C; CARCASSI, C; SCORZA, R; MORAES, JR; MORAES, ME; GORODEZKY, C; FAINBOIM, L; STASTNY, P; FERNANDEZ-VIÑAS, MARCELO

París, Francia.

Conferencia; 12th International Histocompatibility Conference.; 1996

As part of the Anthropology Components, the distribution of HLA-B35 alleles (B*3501 to 35013) was studied in 16 different populations by group specific amplification and SSOP hybridization. The results were as follows: Population: French (65*): 3501 (38), 3502 (5), 3503 (19), 3504 (0), 3505 (0), 3506 (0), 3508 (3), 3509 (0), 35v# (1); Spanish (37*): 3501 (19), 3502 (7), 3503 (7), 3504 (0), 3505 (0), 3506 (0), 3508 (5), 3509 (0), 35v# (0); Greeks (29): 3501 (14), 3502 (2), 3503 (8), 3504 (0), 3505 (0), 3506 (0), 3508 (4), 3509 (0), 35v# (1); N.Ame.Cau (50): 3501 (33), 3502 (4), 3503 (9), 3504 (0), 3505 (0), 3506 (0), 3508 (3), 3509 (0), 35v# (1); Gypsies (17): 3501 (4), 3502 (1), 3503 (12), 3504 (0), 3505 (0), 3506 (0), 3508 (0), 3509 (0), 35v# (0); Ashkenzi Jews (36*): 3501 (13), 3502 (19), 3503 (2), 3504 (0), 3505 (0), 3506 (0), 3508 (4), 3509 (0), 35v# (0); Non-Ashk Jews (17): 3501 (1), 3502 (8), 3503 (3), 3504 (0), 3505 (0), 3506 (0), 3508 (5), 3509 (0), 35v# (0); Arabs (17): 3501 (3), 3502 (4), 3503 (4), 3504 (0), 3505 (0), 3506 (0), 3508 (7), 3509 (0), 35v# (0); N.Ame.Blk (19): 3501 (13), 3502 (1), 3503 (1), 3504 (0), 3505 (0), 3506 (0), 3508 (2), 3509 (0), 35v# (2); Ital.Milano (25): 3501 (13), 3502 (3), 3503 (7), 3504 (0), 3505 (0), 3506 (0), 3508 (2), 3509 (0), 35v# (0); Ital.Sardinian (29*): 3501 (20), 3502 (11), 3503 (0), 3504 (0), 3505 (0), 3506 (0), 3508 (0), 3509 (0), 35v# (0); Cau.Arg. (47*): 3501 (23), 3502 (10), 3503 (9), 3504 (0), 3505 (0), 3506 (0), 3508 (4), 3509 (1), 35v# (1); Mexican Mestizos (24): 3501 (12), 3502 (0), 3503 (0), 3504 (0), 3505 (0), 3506 (0), 3508 (1), 3509 (0), 35v# (12); Mapuches (5*): 3501 (3), 3502 (0), 3503 (0), 3504 (0), 3505 (0), 3506 (0), 3508 (0), 3509 (3), 35v# (0); Chiriguanos (14): 3501 (2), 3502 (1), 3503 (0), 3504 (0), 3505 (0), 3506 (0), 3508 (0), 3509 (0), 35v# (9); Terena (10): 3501 (0), 3502 (0), 3503 (0), 3504 (3), 3505 (1), 3506 (7), 3508 (0), 3509 (1), 35v# (0). * Some individuals carry two different B35 subtypes. # New B35 alleles. The predominant alleles in most Caucasian populations were 3501>3503>3502>3508. However, B*3502 predominated in Jews, B*3508 in Arabs, B*3503 in Gypsies and Sardinians seem to have only B*3501 and 3502. B*3504, 3505, 3506 and 3509 were restricted to Amerindians, where there are still other new B35 variants to be characterized. In most individuals the different B35 alleles were found in phenotypic combinations with HLA-Cw4, suggesting that the B35, Cw4 haplotype may existed before subtype diversification. A detailed analysis of HLA-B35 alleles in other populations might help to draw a precise picture of B35 evolution.