THE VITA-PAMP BRUCELLA ABORTUS RNA MODULATES LYMPHOCYTE RESPONSES

BERTINAT, YASMÍN; BUENO, JORGELINA; SERAFINO, AGUSTINA; MILILLO, M. AYELÉN; BARRIONUEVO, PAULA

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias 2022; 2022

SAIC-SAI-FAIC-SAFIS

B. abortus (Ba), the causative agent of brucellosis, displays many strategies to evade T cell responses conducive to persist inside the host. We have previously showed that Ba can indirectly inhibit T cells by down-regulating MHC expression on macrophages and as a result, antigen presentation. Moreover, we demonstrated that Ba through its lipoproteins (structural component of bacteria) induces apoptosis of T lymphocytes. On the other hand, we have recently demonstrated that Ba RNA (PAMP related to pathogens’ viability or vita-PAMP) is involved in macrophage immune-modulation. However, we did not know if Ba RNA was able to modulate T cell responses. To start with, we incubated cells of JURKAT T cell line with Ba for 1, 2, 3, 5 and 10 days. After each time point, the expression of the senescence marker CD28 was evaluated by flow cytometry. Ba diminished the expression of CD28 at 5 and 10 days (p