Brucella abortus RNA diminishes IFN-gamma-induced MHC-I molecules in different line regular and tumor cells.

SERAFINO, AGUSTINA; BERTINAT, YASMÍN; BUENO, JORGELINA; MILILLO, M AYELÉN; BARRIONUEVO, PAULA

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias 2022; 2022

SAIC-SAI-FAIC-SAFIS

Brucella abortus (Ba) is an intracellular pathogen capable of surviving inside macrophages. Since the disease is presented in multiple forms, many different cells are susceptible to be infected by Ba. We have previously demonstrated that Ba RNA diminishes the IFN-ɤ-induced MHC-I surface expression in human macrophages, retaining them within the Golgi Apparatus (GA). However, we acknowledged whether this event could be triggered in other cells able to be infected with Ba. So, we stimulated the lung epithelium cell line (Calu-6) and the endothelial microvasculature cell line (HMEC) with different doses of Ba RNA in the presence of IFN-ɤ. MHC-I expression was assessed by flow cytometry. Ba RNA (10 ug/ml) diminished the IFN-ɤ-induced MHC-I surface expression (p