The down-modulation of IFN-gamma-induced MHC-I expression by Brucella abortus can be extrapolated to cells other than monocytes.

SERAFINO, AGUSTINA; MILILLO, M. AYELÉN; BARRIONUEVO, PAULA

Buenos Aires

Congreso; Reunión Conjunta SAIC-SAI-AAFE-NANOMED.AR; 2021

SAIC-SAI-AAFE-NANOMED.AR

Brucella abortus (Ba) is an intracellular pathogen capable of surviving inside macrophages. Since the disease is presented in multiple forms, many different cells are susceptible to be infected by Ba. This bug is able to evade the host immune system. We have previously demonstrated that infection of human macrophages with Ba diminishes the IFN-ˠ-induced MHC-I surface expression. One of the PAMPs that triggers this down-modulation is Ba RNA. MHC-I total expression is not altered, instead these proteins are retained within the Golgi Apparatus (GA). However, we acknowledged whether this event could be triggered in other cells able to be infected with Ba. So, we started by stimulating the lung epithelium cell line (Calu-6) and the endothelial microvasculature cell line (HMEC) with different doses of Ba RNA in the presence of IFN-ˠ. MHC-I expression was assessed by flow cytometry. Ba RNA (10 ug/ml) diminished the IFN-ˠ-induced MHC-I surface expression (p