Toxicity Evaluation of Pesticide Mixtures Through Oxidative Stress and Genotoxic Damage in Palaemon argentinus.

GONZALEZ, M.FLORENCIA; BERTRAND, LIDWINA; AMÉ, MARÍA VALERIA

Montevideo

Congreso; SETAC Latin America 15th Biennial Meeting; 2023

SETAC

Aquatic organisms are exposed to multiple stressors, including the insecticide chlorantraniliprole (C), the fungicide epoxiconazole (E), and the herbicide metolachlor (M), which enter water bodies by drift or runoff.The risk associated with exposure to complex mixtures may be underestimated if only the individual toxicity of each of the stressors is taken into account without considering the possible interactions between them.The aim of this work was to expose the native decapod, Palaemon argentinus, to binary and ternarymixtures of C, E and M under laboratory conditions to evaluate the antioxidant activity through catalase activity (CAT), the oxidative damage through carbonylated proteins content (CP) in cephalothorax and abdomen, and establish genotoxic damage using a DNA damage index (DI) measured by comet assay in the decapod hepatopancreas. Individuals were exposed for 96 h (n=8 (CAT), n=6 (CP), n=15 (DI)) to two environmentally relevant concentration levels, low (LC) and high (HC), in equitoxic units: C: 0.002 and 0.04 μgL-1; E: 1.3 and 26.07 μgL-1; M: 3.5 and 70.5 μgL-1 and a control condition (CTL): 0 μgL-1. The results in cephalothorax showed a significant increase (p