Antioxidant, phase II and III responses induced by lipoic acid in the fish Jenynsia multidentata (Anablapidae) and its influence on endolsulfan accumulation and toxicity

MONSERRAT, JOSÉ MARÍA; GARCIA, M.L.; VENTURA LIMA, J.; GONZÁLEZ, M.; BALLESTEROS, M.L.; MIGLIORANZA, K.; AMÉ, MARÍA VALERIA; WUNDERLIN, DANIEL ALBERTO

PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY

ACADEMIC PRESS INC ELSEVIER SCIENCE

Lugar: Amsterdam; Año: 2014 vol. 108 p. 8 - 15

0048-3575

Antioxidants like lipoic acid (LA) are known to trigger augmented antioxidant and phase II and III responses. This study aimed to evaluate the effect of LA in Pgp expression, GST activity, total antioxidant competence, levels of lipid peroxides (TBARS) and accumulation of the organochlorine insecticide endosulfan (Endo: a-, b- isomers and sulfate metabolite) in different organs of the fish Jenynsia multidentata. One hundred and twenty females (1.55 ± 0.07 g) were fed during 8 day with (n=60) or without (n=60) a LA enriched ration (4 g/kg). Four experimental groups were defined: controls (with or without LA pre-treatment) and Endo exposed (with or without LA pre-treatment) during 24 h to 1.4 µg/L. Results showed that LA induced a significant increment in liver Pgp expression, which result not affected by Endo exposure. GST activity was augmented in liver after exposure to LA or Endo. TBARS levels were lowered in liver and gills after LA pre-treatment. Total antioxidant capacity was lowered in liver of Endo exposed fish, a result that was reversed by LA pre-treatment. It is concluded that LA induced the expected effects in terms of Pgp expression, GST activity and reduced TBARS levels although favored a-Endo accumulation in brain. However, the Endo metabolism to the more persistent endosulfan sulfate was not facilitated by LA pre-treatment.