INVESTIGADORES
PATRIARCA Andrea Rosana
congresos y reuniones científicas
Título:
Study of the antioxidant properties and stability of Eucalyptus globulus and Calendula officinalis extracts for synthetic additive replacement
Autor/es:
DOS SANTOS FERREIRA, C.; PATRIARCA, A.; PEREYRA GONZALEZ, A.; ABRAM, V.; POKLAR ULRIH, N.; BUERA, M.P.; MAZZOBRE, F.
Lugar:
Buenos Aires
Reunión:
Congreso; VI International Conference on Polyphenols and Health; 2013
Resumen:
Plant extracts have emerged as sources of natural
antioxidants for food formulations to replace synthetic additives that are currently being questioned by consumers. Eucalyptus leaves,
oil, fruits and barks and marigold flowers have been used in traditional
medicine due to their antinflamatory, antibacterial and analgesic properties.
The aim of this
work was to determine the experimental conditions to maximize the polyphenols
extraction from eucalyptus dried leaves extracts (Eucalyptus Globulus) and from marigold dried flowers (Calendula officialis) and also to analyze
the relationship between polyphenols content and antioxidant activity.
Extracts were obtained by stirring in water-methanol and water-ethanol
solutions. After vacuum evaporation of solvents, extracts were freeze-dried and
stored under nitrogen atmosphere. UV-Vis spectra of samples were determined in
the range 800-200 nm. Total phenolic
content (TPC) was evaluated by Folin?Ciocalteu and the antioxidant capacity
(AOC) by HPLC with electrochemical detector and by DPPH assay. The
maximum polyphenol content was achieved in methanol-water 70:30. A direct correlation
between the amount of TPC and AOC was
observed for all the analyzed extracts.
Eucalyptus showed higher absorbance at 300-270 nm, and
higher TPC contents
and AOC than marigold extracts.
Eucalyptus extracts could be sources of antioxidants to employ in the
formulation of functional ingredients.