IIB   20738
INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
artículos
Título:
Reassessing the role of phospholipase D in the Arabidopsis wounding response
Autor/es:
BARGMANN, B.; LAXALT, A.M.; TER RIET, B.; TESTERINK, C.; MERQUIOL, E.; MOSBLECH, A.; LEON REYES, A.; PIETERSE, C.M.J.; HARING, M.; HEILMANN, I; BARTELS, D.; MUNNIK, T.
Revista:
PLANT, CELL AND ENVIRONMENT (PRINT)
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2009 vol. 32 p. 837 - 850
ISSN:
0140-7791
Resumen:
Plants respond to wounding by means of a multitude of
reactions, with the purpose of stifling herbivore assault.
Phospholipase D (PLD) has previously been implicated
in the wounding response. Arabidopsis (Arabidopsis
thaliana) AtPLDa1 has been proposed to be activated in
intact cells, and the phosphatidic acid (PA) it produces to
serve as a precursor for jasmonic acid (JA) synthesis and
to be required for wounding-induced gene expression.
Independently, PLD activity has been reported to have a
bearing on wounding-induced MAPK activation. However,
which PLD isoforms are activated, where this activity takes
place (in the wounded or non-wounded cells) and what
exactly the consequences are is a question that has not been
comprehensively addressed. Here, we show that PLD activity
during the wounding response is restricted to the ruptured
cells using 32Pi-labelled phospholipid analyses of
Arabidopsis pld knock-out mutants and PLD-silenced
tomato cell-suspension cultures. plda1 knock-out lines
have reduced wounding-induced PA production, and the
remainder is completely eliminated in a plda1/d double
knock-out line. Surprisingly, wounding-induced protein
kinase activation, AtLOX2 gene expression and JA biosynthesis
were not affected in these knock-out lines.Moreover,
larvae of the Cabbage White butterfly (Pieris rapae) grew
equally well on wild-type and the pld knock-out mutants.