IIB 20738
INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
artículos
Título:
The carbonic anhydrase domain of plant mitochondrial complex I
Autor/es:
ZABALETA, EDUARDO; FROMM, STEFFANIE; PETERHANSEL, CHRISTOPHE; SENKLER, JENNIFER; BRAUN, HANS PETER
Revista:
PHYSIOLOGIA PLANTARUM
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2016 vol. 157 p. 283 - 296
ISSN:
0031-9317
Resumen:
The mitochondrial NADH dehydrogenase complex (complex I) consists of several functional domains which independently arose during evolution. In higher plants, it contains an additional domain which includes proteins resembling gamma-type carbonic anhydrases. The Arabidopsis genome codes for five complex I-integrated gamma-type carbonic anhydrases (γCA1, γCA2, γCA3, γCAL1, γCAL2), but an individual extra domain only includes three copies of this group of proteins. Biochemical analyses revealed that the domain is composed of one copy of either γCAL1 or γCAL2 plus two copies of the γCA1/γCA2 proteins. Thus, the carbonic anhydrase domain can have six distinct subunit configurations. Single and double mutants with respect to the γCA/γCAL proteins were employed to genetically dissect the function of the domain. New insights into complex I biology in plants will be reviewed and discussed.
