Native isolates of Trichoderma harzianum inducting resistance to Zymoseptoria tritici on wheat plants

STOCCO, M; MANSILLA, A.Y.; MONACO, C.; SEGARRA, C.I.; LAMPUGNANI, G.; ABRAMOFF, C.; MARCHETTI, M.F.; KRIPELZ, N; CORDO, C.; CONSOLO, V.F.

BOLETíN DE LA SOCIEDAD ARGENTINA DE BOTáNICA

SOCIEDAD ARGENTINA DE BOTÁNICA

Lugar: Córdoba; Año: 2015 vol. 3 p. 291 - 301

0373-580X

Trichoderma harzianum inducting resistance to Zymoseptoria tritici. Septoria tritici blotchis endemic in the wheat growing cultivated areas of Argentina, which impacts in the crop yield. Onemanagement strategy is to use biocontrol agents. The objective of this work was to determine theeffectiveness of Trichoderma spp. isolated from soils of different growing regions, as antagonist andcharacterize the T. harzianum effect throughout the proteolytic activity of the serine protease in the wheatplant. To determine the antagonistic capacities of Trichoderma spp. against Zimoseptoria tritici, wheatplants were grown from seeds coated with each of the ninety isolated Trichoderma. Antagonism wasassessed 21 days after inoculation by the capacity of each Trichoderma spp. isolate to restrict the progressof the disease. To measure the increasing proteolytic activity, ten isolated T. harzianum characterized bytheir inhibitory effect over the pathogen, were chosen. Four isolates of the antagonist (strain 2 and 8, fromLos Hornos and 129 and 141 from Manfredi localities) simultaneously decreased the pycnidial coveragepercentage with a noted increasing of the serine-protease activity in the susceptible wheat cultivar. Thisbehavior is associated to a biochemical mechanism of defense with an early systemic effect.