Molecular characterization of Echinococcus granulosus isolates from cattle, pig, sheep and human from central region of Argentina

ANDRESIUK M.V., PONCE-GORDO F., CASALONGUÉ C., ELISSONDO M.C., TARABORELLI A., DOPCHIZ M., DENEGRI G.

París, Francia

Congreso; X European Multicolloquium of Parasitology; 2008

Societe Francaise de Parasitologie

Cystic Echrnococcosrs ts endemic in Argentina. To study tbe disease and to design control programs is necessary (among cther things) the charactertzatton of the genotypes which differ in sorne epidemiological features. As a consequence of the diverse geographic and c1imatic conditions the characterization e important in every Argentinean region. Central region of the country presents an intense farm actlvlty where there are several types of intermediary hosts allowing tbe establishment ot more than one epidemiological chain. The aim 01 this work was to determine the genotypes of Echinococcus cremnosus in cattle, pig, sheep and human from Central Region of Argentina. A total of 142 isolates were recovered. ONA extracüon was carried out by standard protocols. PCRs with primers for regions of the C01 and N01 mitochondria! genes were performed. Sixty five isolates of cattle orlqln belonged to the G1 genotype (common sheep strain), 35 isolates trom sheep belonged to the same genotype and also 5 isolates from humans. 01 a whole of 37 isolates from pigs, 17 belonged to G7 (pig strain) and the rest to G1. Within each genotype several micro variants were recorded: 3 for C01 and 4 lor N01. This report contríbutes to the cheracterízatlon of the E. granulosus genotypes that exisl in the region and also 10 the knowledge of the disease epidemiology for the Central region of Argentina.