Effects of ovarian hyperstimulation on early embryo development: a study in a murine model.

RAMÍREZ ND; TORRES PJ; LUQUE EM; PONZIO MF; CANTARELLI V; MOTRICH RD; MARTINI AC

CORDOBA

Jornada; XXIII Jornada de Investigación de la Facultad de Ciencias Médicas; 2022

Controlled ovarian hyperstimulation (COH) is a protocol commonly applied in assisted reproduction techniques. It involves the pharmacological stimulation of the ovaries, in order to increase the number of oocytes recovered and fertilization probabilities. However, the concomitant increase in sexual steroid levels, are thought to negatively impact on embryo development. The aim of this study was to assess the possible impact of COH on ovulation, fertilization and preimplantation embryo development in a mouse model.Albino Swiss (N:NIH) adult female mice were treated (COH group) with human menopausic gonadotropin and human chorionic gonadotropin (10 I.U/day each), and oocytes number and quality were recorded. A second COH group was mated with untreated males, and their uteri were removed (three days post coitus), in order to asses embryos/oocytes number and developmental stage (2-8 cells, early/late morula and compact/expanded blastocyst). The percentage of fertilization was calculated considering embryo and corpora lutea numbers. Females in natural estrous were used as the control group (C).Results were analyzed by T/Mann-Whitney test; in all cases p