NANOPARTICLES FOR CARIES LESION INFILTRATION AND POTENTIAL IN SITU REPAIRMENT

SILVERO C., M. JAZMÍN; ANGEL VILLEGAS N; MARTIN SAINZ AJA; ROCCA D; BECERRA M C; MOLINA, GUSTAVO; PALMA S

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Congreso; Reunion conjunta de sociedades de biociencias; 2017

Caries disease produces serious dental damage thateventually evolves in a tooth cavity. The aim of this work was to study theapplication of nanomaterials to infiltrate decayed tissue and promote abiological repairment response. Zinc nanoparticles (ZnO@NP, 50nm) and goldnanoparticles synthesized and stabilized with an antibiotic (amoxicillin@AuNP,40nm) were selected for their known antimicrobial capacity andbiocompatibility. ZnO@NP produced stable suspensions during 72h in: distilled H2O,PBS (pH=7.4), Thioglycolate broth, DMEM and IconR (a resin-basedmaterial mainly composed of tri-ethylene glycol dimethacrylate with highinfiltration ability). The amoxicillin@AuNP stability, followedspectrophotometrically, was acceptable in DMEM and Thioglycolate broth (12.5%)​​for 72h, and was excellent in the resin until 72h. Agregation was notedimmediately when mixing the two nanomaterials in brain-heart broth and in H2O2(3%), which were alternatives for the culture of anaerobic bacteria and forinfiltration, respectively. The interaction between nanomaterials and thedemineralized dental surface was analyzed by SEM (Surface Electron Microscope)with coupled EDS (Energy Dispersive X-Ray Spectrometer). The load ofnanomaterial was 22 times higher (Wt%: 42.9 ± 1) in dental samples treated withZnO@NP carried in resin, than in those treated with ZnO@NP at the sameconcentration in PBS (Wt%: 1.9 ± 1). SEM images with mapping also showed thatamoxicillin AuNP (1.5 μg/mL in PBS 50%) were attached on the dental surface,preferably in the demineralized area. Overall, best results were obtained byemploying the resin as a carrier.  Thisadhesive system based on methacrylates improved the nanomaterial adherence andrecovered the dental structure by filling the porosities of the simulatedlesion. Currently, their infiltration power and their effect on cariesproducing bacterial strains are being investigated.