CYCLIC-DI-GMP SIGNALING REGULATES MOTILITY AND BIOFILM FORMATION IN BORDETELLA BRONCHISEPTICA

SISTI, FEDERICO; HA, DAE GON; O´TOOLE, GEORGE A; HOZBOR, DANIELA; FERNANDEZ, JULIETA

MICROBIOLOGY-UK

SOC GENERAL MICROBIOLOGY

Lugar: London; Año: 2013 vol. 159 p. 869 - 879

1350-0872

The signaling molecule 3´,5´-cyclic diguanylic acid (c-di-GMP) is a central regulator of diverse cellular functions, including motility, biofilm formation, cell cycle progression, and virulence in bacteria. Multiple diguanylate cyclase and phosphodiesterase domain-containing proteins (GGDEF and EAL/HD-GYP, respectively) modulate the levels of the second messenger c-di-GMP to transmit signals and obtain such specific cellular responses. In Bordetella genus this c-di-GMP network is poorly studied. In this work, we evaluated the expression of two phenotypes in Bordetella bronchiseptica regulated by c-di-GMP, biofilm formation and motility, under the ectopic expression of Pseudomonas aeruginosa proteins with EAL or GGDEF domain that regulates the c-di GMP level. In agreement with previous reports for other bacteria, we observed that B. bronchiseptica is able to form biofilm and reduce its motility only when GGDEF domain protein is expressed. Moreover we identify a GGDEF domain protein (BB3576) with diguanylate cyclase activity that participates in motility and biofilm regulation in B. bronchiseptica. These results demonstrate for the first time the presence of c-di-GMP regulatory signaling in B. bronchiseptica.