INVESTIGADORES
HALLAK Marta Elena
artículos
Título:
Astrocytes and oligodendrocytes express different STOP proteins isoforms
Autor/es:
GALIANO, M. R.; BOSC C.; ANDRIEUX A., JOB, D. & HALLAK, M.
Revista:
JOURNAL OF NEUROSCIENCE RESEARCH
Referencias:
Año: 2004 p. 329 - 337
ISSN:
0360-4012
Resumen:
Many cell types contain subpopulations of microtubules
that resist depolymerizing conditions, such as exposure
to cold or to the drug nocodazole. This stabilization is
due mainly to polymer association with STOP proteins. In
mouse, neurons express two major variants of these
proteins, N-STOP and E-STOP (120 kDa and 79 kDa,
respectively), whereas fibroblasts express F-STOP (42
kDa) and two minor variants of 48 and 89 kDa. N- and
E-STOP induce microtubule resistance to both cold and
nocodazole exposure, whereas F-STOP confers microtubule
stability only to the cold. Here, we investigated the
expression of STOP proteins in oligodendrocytes and
astrocytes in culture. We found that STOP proteins were
expressed in precursor cells, in immature and mature
oligodendrocytes, and in astrocytes. We found that oligodendrocytes
express a major STOP variant of 89 kDa,
which we called O-STOP, and two minor variants of 42
and 48 kDa. The STOP variants expressed by oligodendrocytes
induce microtubule resistance to the cold and
to nocodazole. For astrocytes, we found the expression
of two STOP variants of 42 and 48 kDa and a new STOP
isoform of 60 kDa, which we called A-STOP. The STOP
variants expressed by astrocytes induce microtubule resistance
to the cold but not to nocodazole, as fibroblast
variants. In conclusion, astrocytes and oligodendrocytes
express different isoforms of STOP protein, which show
different microtubule-stabilizing capacities.
variants expressed by astrocytes induce microtubule resistance
to the cold but not to nocodazole, as fibroblast
variants. In conclusion, astrocytes and oligodendrocytes
express different isoforms of STOP protein, which show
different microtubule-stabilizing capacities.
and 48 kDa. The STOP variants expressed by oligodendrocytes
induce microtubule resistance to the cold and
to nocodazole. For astrocytes, we found the expression
of two STOP variants of 42 and 48 kDa and a new STOP
isoform of 60 kDa, which we called A-STOP. The STOP
variants expressed by astrocytes induce microtubule resistance
to the cold but not to nocodazole, as fibroblast
variants. In conclusion, astrocytes and oligodendrocytes
express different isoforms of STOP protein, which show
different microtubule-stabilizing capacities.
variants expressed by astrocytes induce microtubule resistance
to the cold but not to nocodazole, as fibroblast
variants. In conclusion, astrocytes and oligodendrocytes
express different isoforms of STOP protein, which show
different microtubule-stabilizing capacities.
O-STOP, and two minor variants of 42
and 48 kDa. The STOP variants expressed by oligodendrocytes
induce microtubule resistance to the cold and
to nocodazole. For astrocytes, we found the expression
of two STOP variants of 42 and 48 kDa and a new STOP
isoform of 60 kDa, which we called A-STOP. The STOP
variants expressed by astrocytes induce microtubule resistance
to the cold but not to nocodazole, as fibroblast
variants. In conclusion, astrocytes and oligodendrocytes
express different isoforms of STOP protein, which show
different microtubule-stabilizing capacities.
variants expressed by astrocytes induce microtubule resistance
to the cold but not to nocodazole, as fibroblast
variants. In conclusion, astrocytes and oligodendrocytes
express different isoforms of STOP protein, which show
different microtubule-stabilizing capacities.
A-STOP. The STOP
variants expressed by astrocytes induce microtubule resistance
to the cold but not to nocodazole, as fibroblast
variants. In conclusion, astrocytes and oligodendrocytes
express different isoforms of STOP protein, which show
different microtubule-stabilizing capacities.